Technical Data
I7663-08W
Interleukin 1 beta, Recombinant, Chicken (Interleukin-1beta, IL1b, IL-1 beta, Il-1beta) (Azide Free)
50ug
Molecular Biology Storage: -20CShipping: Blue Ice
Interleukin1 (IL1) is a proinflammatory cytokine secreted by many different cell types which acts upon a wide range of target cells, resulting in a diverse range of effects. The biological properties of IL1 include the induction of B cell proliferation, stimulation of T cell proliferation in conjunction with IL2 and the production of collagenase and prostaglandin by fibroblasts. IL1 is also responsible for triggering the acutephase response in the liver, and is involved in both cytokine network and vascular endothelium activation.

I7663-08W induces corticosterone secretion in vivo(1) and is the immunogen used to produce chicken IL1beta antibody I7663-08V,

Source:
Recombinant corresponding to chicken IL-1 beta, histagged, expressed in E.coli.

Molecular Weight:
~23kD

Applications:
Suitable for use in Functional Assays and Immunoassays. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: Chicken
Purity: Purified: ~85% (Ni chelate Chromatography)
Concentration: ~0.25mg/ml
Form: Supplied as a liquid in PBS. Preservative free.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Weining, K.C. et al. (1998) A chicken homolog of mammalian interleukin1 beta: cDNA cloning and purification of active recombinant protein. Eur. J. Biochem. 258: 9941000. 2. Gyorfy, Z. et al. (2003) Truncated chicken interleukin1 beta with increased biologic activity. J. Interferon and Cytokine Research 23: 223228.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.