Technical Data
I7663-25Q
Interleukin 1 Receptor Antagonist (IL-1Ra)
Description:
Intracellular Interleukin 1 Receptor Antagonist (icIL-1Ra) is found in many cells as a constitutively expressed protein, particularly epithelial cells and keratinocytes.
Biologically, secreted Interleukin 1 Receptor Antagonist (sIL-1Ra) and icIL-1Ra both function as IL-1 receptor antagonists by binding to the type I IL-1R and preventing the signaling by IL-1a or IL-1b. However, sIL-1Ra is primarily secreted whereas icIL-1Ra remains associated with the cell.

Applications:
Suitable for for use in Neutralization, ELISA, RIA, Western Blot, Immunohistochemistry and Flow Cytometry. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:1000; Using I1904-46Q and ABTS as a substrate for 30 minutes at RT.
RIA: 1:8000
Western Blot: 1:400-1:800; Using I1904-46Q and TMB as a substrate.
Immunohistochemistry (paraffin-fixed/ frozen): 1:400
Neutralization: 1:200 will neutralize the inhibitory activity of IL-1Ra at 100ng/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
1mg-20°CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Recombinant human sIL-1Ra produced in E. coli.
Purity:
Purified by DEAE chromatography. Endotoxin Content: < 10pg/ul by LAL method.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Interleukin 1 Receptor Antagonist. Detects primate IL-1Ra in the same formats using similar dilutions, but not mouse or rabbit IL-1Ra. Does not recognize human IL-1a, IL-1b or IL-1 receptor type I and type II. Primarily directed against the secreted form of IL-1Ra (also termed sIL-1Ra), but recognizes the native (non-denatured) as well as denatured intracellular form of IL-1Ra (also termed icIL-1Ra).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arend, W.P., Malyak, M., et al., Ann. Rev. Immunol. 16: 27-55 (1998). 2. Eisenberg, S.P., Evans, R.J., et al., Nature 343: 342-346 (1990). 3. Hammerberg, C., Arend, W.P., et al., J. Clin. Invest. 90: 571-583 (1992). 4. Haskill, S., Martin, M., et al., Proc. Natl. Acad. Sci. USA 88: 3681-3685 (1991).