Technical Data
I7663B
Interleukin 1 alpha, Recombinant, Human (Interleukin-1 alpha, IL-1 alpha, IL1-alpha, IL1A, IL-1A, Hematopoietin-1, IL1F1)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Source:
Recombinant corresponding to 159aa of human Interleukin-1 alpha expressed in E.coli is a single, non-glycosylated, polypeptide chain.

Molecular Weight:
~18kD

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of mouse D10S cellsis less then 0.001ng/ml, corresponding to a Specific Activity of 1x10 IU/mg.

Dimers and Aggregates:
1% (SDS-PAGE)

Protein Content:
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~18kD
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug)
Concentration: ~1mg/ml
Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Human interleukin-1 alpha gene expression is regulated by Sp1 and a transcriptional repressor. Cytokine 2005 May 21;30(4):141-53. 2. Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha. Stem Cells 2005 Mar;23(3):383-91. 3. Effect of early luteal phase administration of a single dose mifepristone on immunohistochemical distribution of interleukin 1 alpha (IL-1 alpha) and transforming growth factor beta 1 (TGF-beta 1) in mid-luteal phase ovary of the rhesus monkey. Indian J Physiol Pharmacol 2003 Apr;47(2):221-4. 4. [Analysis on association between the polymorphisms in apolipoprotein E, interleukin-1 alpha genes and Alzheimer's disease in Chengdu area]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2004 Apr;21(2):176-8. 5. [Content of tumor necrosis factor alpha and interleukin 1-alpha in patients with acute purulent pleural cavity]. Klin Khir 2003 Dec;(12):26-8. 6. Lack of association between the interleukin-1 alpha (-889) polymorphism and early-onset Parkinson's disease. Neurosci Lett 2004 Apr 15;359(3):195-7.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.