Technical Data
I7663B
Interleukin 1 alpha, Recombinant, Human (Interleukin-1 alpha, IL-1 alpha, IL1-alpha, IL1A, IL-1A, Hematopoietin-1, IL1F1)
2ug
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Technical Data |
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I7663B |
Interleukin 1 alpha, Recombinant, Human (Interleukin-1 alpha, IL-1 alpha, IL1-alpha, IL1A, IL-1A, Hematopoietin-1, IL1F1) |
2ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to 159aa of human Interleukin-1 alpha expressed in E.coli is a single, non-glycosylated, polypeptide chain. Molecular Weight: ~18kD Biological Activity: The ED50 as determined by the dose-dependent stimulation of mouse D10S cellsis less then 0.001ng/ml, corresponding to a Specific Activity of 1x10 IU/mg. Dimmers and Aggregates: 1% (SDS-PAGE) Protein Content: Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280nm using the absorbency value of 1.13 as the extinctioncoefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENEcomputer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of chicken IL-1alpha as a Reference Standard. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O, HSA or BSA. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~18kD |
Purity: ~98% (RP-HPLC, Anion-exchange FPLC, SDS-PAGE) Endotoxin: 0.1ng/ug (IEU/ug) Concentration: ~1mg/ml Form: Supplied as a lyophilized powder. No preservative added. Reconstitute with sterile dH2O, 0.1% HSA or BSA to 0.1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Human interleukin-1 alpha gene expression is regulated by Sp1 and a transcriptional repressor. Cytokine 2005 May 21;30(4):141-53. 2. Neurons derived from human mesenchymal stem cells show synaptic transmission and can be induced to produce the neurotransmitter substance P by interleukin-1 alpha. Stem Cells 2005 Mar;23(3):383-91. 3. Effect of early luteal phase administration of a single dose mifepristone on immunohistochemical distribution of interleukin 1 alpha (IL-1 alpha) and transforming growth factor beta 1 (TGF-beta 1) in mid-luteal phase ovary of the rhesus monkey. Indian J Physiol Pharmacol 2003 Apr;47(2):221-4. 4. [Analysis on association between the polymorphisms in apolipoprotein E, interleukin-1 alpha genes and Alzheimer's disease in Chengdu area]. Zhonghua Yi Xue Yi Chuan Xue Za Zhi 2004 Apr;21(2):176-8. 5. [Content of tumor necrosis factor alpha and interleukin 1-alpha in patients with acute purulent pleural cavity]. Klin Khir 2003 Dec;(12):26-8. 6. Lack of association between the interleukin-1 alpha (-889) polymorphism and early-onset Parkinson's disease. Neurosci Lett 2004 Apr 15;359(3):195-7.
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