Technical Data
I8432-01
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Description:
Interleukins (ILs) are a large group of cytokines that are produced mainly by leukocytes, although some are produced by certain phagocytes and auxiliary cells. ILs have a variety of functions, but most function to direct other immune cells to divide and differentiate. Each IL acts on a specific, limited group of cells through a receptor specific for that IL. Human IL10 is a non-glycosylated polypeptide consisting of 160 amino acids. The cellular sources of IL10 are CD4+ T cells and T cell clones, thymocytes, B cells and B cell lymphomas, macrophages, mast cell lines, and keratinocytes. IL10 will stimulate the growth of stem cells, mast cells, and thymocytes.

Applications:
Suitable for use in ELISA and immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (Frozen): 10-20ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil tissue

Hybridoma:
BALB/c mouse spleen cells were fused with SP/20 mouse myeloma cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k5J47Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Purified recombinant human IL-10
Purity:
Purified by Protein A affinity column chromatography.
Form
Supplied as a liquid in PBS, pH 7.5, 0.05% sodium azide.
Specificity:
Recognizes natural and recombinant human IL-10. Cross-reactivity: Epstein-Barr viral IL-10.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Van Hoffen, E., D. van Wichen, I. Stuij, N. de Jonge, C. de Klopping, J. Lahpor, J. van den Tweel, F. Gmelig-Meyling, and R. de Weger (1996) In situ expression of cytokines in human heart allografts. American Journal of Pathology 149:1991-2003. 2. Hong, Y.S., H. Laks, G.G. Cui, T. Chong, and L.Y. Sen (2002) Localized immunosuppression in the cardiac allograft induced by a new liposome-mediated IL-10 gene therapy. J. Heart Lung Transplantation 21(11):1188-1200.