Technical Data
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Molecular Biology Storage: -70CShipping: Dry Ice
Recombinant corresponding to human Interleukin 10 expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50=0.2-1ng/ml (1-5x10e6units/mg). The biological activity was determined by measuring the dose-dependent proliferation of murine MC/9 cells. Optimal concentration for individual application should be determined by an initial dose response assay.

Storage and Stability:
Lyophilized powder may be stored at -70C. Reconstitute to nominal volume by adding ddH2O and carrier protein. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -27C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute in sterile ddH2O and carrier protein to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Eisenstein, E.M., Chua, K., Strober, W. (1994) J. Immunol. 152(12):5957-5968. 2. Hempel, L., Korholz, D., Bonig, H., Schneider, M., Klein-Vehne, A., Packeisen, J., Mauz-Korholz, C., Burdach, S. (1995) J. Immunol. 41(5):462-466. 3. Miagkov, A.V., A.W. Varley, R.S. Munford, and S.S. Makarov (2002) J. Clin. Invest. 109 (9):1223-1229. 4. Voburka, Z., V. Vetvicka, J. Vetvickova, and M. Fusek (2002) Anticancer Res. 22(2A):913-919.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.