Technical Data
I8432-11B
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
5ug
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Technical Data |
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I8432-11B |
Interleukin 10, Recombinant, Human (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF) |
5ug |
| Growth Factors, Cytokines | Storage: -70°CShipping: Dry Ice |
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Source: Recombinant corresponding to human Interleukin 10 expressed in E. coli. Molecular Weight: ~18.6kD Endotoxin: ~0.1ng/ug Biological Activity: ED50=0.2-1ng/ml (1-5x10e6units/mg). The biological activity was determined by measuring the dose-dependent proliferation of murine MC/9 cells. Optimal concentration for individual application should be determined by an initial dose response assay. Storage and Stability: Lyophilized powder may be stored at -70°C. Reconstitute to nominal volume by adding ddH2O and carrier protein. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -27°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 18.6kD |
Source: E. coli Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute in sterile ddH2O and carrier protein to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Eisenstein, E.M., Chua, K., Strober, W. (1994) J. Immunol. 152(12):5957-5968. 2. Hempel, L., Korholz, D., Bonig, H., Schneider, M., Klein-Vehne, A., Packeisen, J., Mauz-Korholz, C., Burdach, S. (1995) J. Immunol. 41(5):462-466. 3. Miagkov, A.V., A.W. Varley, R.S. Munford, and S.S. Makarov (2002) J. Clin. Invest. 109 (9):1223-1229. 4. Voburka, Z., V. Vetvicka, J. Vetvickova, and M. Fusek (2002) Anticancer Res. 22(2A):913-919.
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