Technical Data
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Direct ELISA: 1:20,000 dilution will yield >1.0 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.
Immunohistochemistry: Intracellular staining
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG15C68Highly Purified
500ug-20CBlue IceHumanMouse
Recombinant human IL-10
Purified by ion exchange chromatography.
Supplied as a liquid in PBS, pH 7.2.
Recognizes human IL-10.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.