Technical Data
I8432-12
Interleukin 10 (IL-10, Cytokine Synthesis Inhibitory Factor, CSIF)
Description:
IL-10 is a cytokine produced primarily by monocytes and to a lesser extent by lymphocytes. This cytokine has pleiotropic effects in immunoregulation and inflammation. It down-regulates the expression of Th1 cytokines, MHC class II antigens, and costimulatory molecules on macrophages. It also enhances B cell survival, proliferation, and antibody production. This cytokine can block NF-kappa B activity, and is involved in the regulation of the JAK-STAT signaling pathway. Knockout studies in mice suggested the function of this cytokine as an essential immunoregulator in the intestinal tract.

Applications:
Suitable for use in Direct ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Direct ELISA: 1:20,000 dilution will yield >1.0 O.D. using alkaline phosphatase conjugated rabbit anti-mouse Ig.
Immunohistochemistry: Intracellular staining
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG15C68Highly Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant human IL-10
Purity:
Purified by ion exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.2.
Specificity:
Recognizes human IL-10.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.