Technical Data
I8434-29J
IL12A (NK Cell Stimulatory Factor Chain 1, NKSF1, Cytotoxic Lymphocyte Maturation Factor 35kD Subunit, CLMF p35, IL-12 Subunit p35, Interleukin-12 Subunit alpha, IL-12A)
Description:
Interleukin 12A is a novel signaling cytokine belonging to the Interleukin-6 protein superfamily. Interleukin-12 alpha chain precursor (IL-12A) is a 35kD subunit of disulfide-linked heterodimer of cytokine IL-12 and has a broad array of biological activities. Reports suggest that Interleukin 12A can act as a growth factor for activated T and NK cells and enhance the lytic activity of NK:lymphokine-activated Killer cells. It stimulates the production of IFN-gamma by resting PBMC as well as T-cell-independent induction of interferon (IFN)-gamma, and helps in the differentiation of both Th1 and Th2 cells. It is also involved in positive regulation of tyrosine phosphorylation of Stat4 protein. The signaling process of IL-12A in innate immunity requires nitric oxide synthase 2A. Along with EBI3 it forms a novel heterodimeric cytokine, designated as interleukin-35 (IL-35), that may be specifically produced by T reg cells and is required for maximal suppressive activity.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
IL-35 recombinant protein lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa200-250 of p35/IL-12A.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human IL12A. Species Crossreactivity: rhesus monnkey. Species sequence homology: chimpanzee, canine, goat, porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liebau, C. et al. Cytokines Cell Mol. Ther. 7: 135-142 (2002). 2. Ye, ZB. et al. World J Gastroenterol. 13: 1747-1751 (2007). 3. Diefenbach, A. et al. Science 284: 951-955 (1999). 4. Kassim, SK. et al. Clin. Biochem. 35: 369-375 (2002).