Technical Data
Internexin, alpha, CT
alpha-Internexin is a Class IV intermediate filament subunit expressed very early in neuronal development. It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. Many classes of mature neurons contain a-internexin in addition to NF-L, NF-M and NF-H. In some mature neurons a-internexin is the only neurofilament subunit expressed. Antibodies to internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of a-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage.

Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and ELISA . Other applications have not been tested.

Recommended Dilutions:
Immunocytochemistry: PC12 and Ntera-2 cells
Immunohistochemistry: Paraffin-embedded, formalin and methonal fixed tisues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
300ul-20CBlue IceRatMouse
Not Determined
Full length recombinant rat alpha internexin
Supplied as a liquid. Contains no preservative.
Recognizes the C-terminal Tail extension of rat alpha internexin. Species Crossreactivity: human, mouse, feline, rabbit, bovine and porcine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Evans, J., et al., J. Neurophysiol. (2002) 87:10761085.