Technical Data
I8447-25
Involucrin
Description:
Involucrin is expressed in a range of stratified squamous epithelia, including the cornea. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is abnormal in squamous cell carcinomas and premalignant lesions and is reduced in severe dysplasias of the larynx and cervix.

Applications:
Suitable for use in ELISA (For coating, use antibody without BSA), Immunofluorescence, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 2ug/mg protein lysate (Denatured verified; Use Protein G).
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 1:100-1:200 for 20 minutes at RT. For staining formalin-fixed tissues, digest sections with trypsin at 1mg/ml PBS, for 10 minutes at 37C or Protease XXV at 1mg/ml PBS, for 5 minutes at 37C.
Note: Enzyme digestion is better than citrate buffer based epitope unmasking.
Optimal dilutions to be determined by the researcher.

Positive Control:
LS174T cells and skin

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.341Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Involucrin from human keratinocytes. MW of Antigen: 66-170kD. Cellular Localization: Cytoplasmic. The epitope maps between residues 421-568 of human involucrin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See I8447-25X.
Specificity:
Recognizes human involucrin at 66-170kD, depending on cell type. Stains involucrin in a variety of sizes: 170kD in MCF-7 cells, a doublet of ~115kD and 150kD in gorilla and owl monkey, 66kD in canine, and a doublet of 105kD in porcine. Species Crossreactivity: gorilla, owl monkey, porcine, canine and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hudson, D.L., et al., Hybridoma 1992, 11:367