Technical Data
Iso-Sensitest Agar
Molecular Biology Grade
Storage 4C    Shipping RT
Components shown as g/liter
Cobalt (II) sulfate0.001
Copper (III) sulfate0.001
Iron (II) sulfate0.001
Manganese (II) chloride0.002
Sodium acetate1.0
Sodium chloride3.0
Sodium phosphate dibasic2.0
Zinc sulfate0.001
Calcium gluconate0.1
Magnesium glycerophosate0.2
Hydrolyzed casein11.0
Soluble starch 1.0
L-Cysteine hydrochloride0.02
Sodium Pantothenate0.003
Total:31.40g/ liter
Iso-Sensitest Agar is a medium developed specifically for anti-microbial susceptibility tests like the Disc Diffusion Method. This is a well-defined medium containing a stabilized mineral content to avoid antagonism to certain antimicrobials by metal ions. It allows the growth of the great majority of microorganisms without further supplementation. It can also be supplemented for fastidious organisms with 5% whole horse blood or 5% whole horse blood and 20mg/l beta-nicotinamide adenine dinucleotide (NAD). Some mutant strains which are totally dependent on thymine and thymidine for their growth will not grow on Iso-Sensitest Agar which has these two components at very low levels. Care must be taken to recognize these strains.

Certain supplements interefere with anti-microbial activity and tests must be performed to measure their effect. These supplements and the anti-microbial agents affected (in parentheses) include: thymidine (trimethoprim), blood (sulfonamides, trimethoprim, aminoglycosides), carbon dioxide (aminoglycosides, erythromycin, lincomycin, tetracycline, novobiocin), cysteine and other sulfhydryl compounds (aminoglycosides), and Vitox/Isovitalex (sulfonamides, trimethoprim).

The addition of lysed horse erthrocytes to the medium is not required when carrying out anti-microbial susceptibility tests with trimethoprim and sulfonamides

Directions per Liter: Suspend 31.4 grams in one liter distilled water and bring to boil to dissolve the agar. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Dehydrated Appearance: Straw colored, homogenous, free flowing powder.

Appearance of Prepared Medium: Straw colored gel

pH: As reprorted

Microbiological Testing: Escherichia coli ATCC 25922

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

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