Technical Data
JNK2 alpha2, active, Recombinant, Human (c-Jun N-terminal Kinase 2, JNK2, JNK2alpha, JNK2A, JNK-55, Mitogen-activated Protein Kinase 9, MAP Kinase 9, MAPK 9, MAPK9, PRKM9, p54a, p54aSAPK, Stress-activated Protein Kinase 1a, SAPK1a, SAPK, Stress-activated
Molecular Biology Storage: -70CShipping: Dry Ice
N-terminal His-tagged, full-length human JNK2a2/SAPK1a expressed in Sf21 cells. Accession number L31951.

JNK2a2 Kinase Assay: JNK2a2 activity was measured using GST-ATF2 as substrate in the kinase assay.

Specific Activity: 80u/mg where one unit of JNK2a2 activity is equal to 1nmol phosphate incorporated into GST-ATF2 per minute.

Molecular Weight:

MS Tryptic Fingerprint:Confirmed as JNK2alpha2/SAPK1a with sequence coverage from 11 peptides, 117-424 aa.

Storage and Stability: Stable for 6 months at -70C from date of shipment.

Molecular Weight:
Source: Recombinant human

Purity: 90%. Purified using Ni-NTA agarose. Mr ~51kD (SDS-PAGE and Coomassie Stain)

Form: Supplied as a frozen solution in 60ul of 50mM Tris-HCl, pH 7.5, 270mM sucrose, 150mM NaCl, 0.1mM EGTA, 0.1% 2-mercaptoethanol, 0.2mM PMSF, 0.03% Brij-35, 1mM benzamidine

Intended for research use only, not for use in human, therapeutic or diagnostic applications.

1. Gupta, S., et al., EMBO J. 15: 27602770, 1996. 2. Gupta, S., et al., Science 267: 389393, 1995. 3. Lawler, S., et al., FEBS Lett 414: 153158, 1997.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.