		Technical Data
J8009-30	Junctional Adhesion Molecule 4 (JAM4, Immunoglobulin Superfamily Member 5, IGSF5, Cortical Thymocyte Marker in Xenopus, CTX) (Biotin)

Description:
JAM-4, also known as IGSF5, is a 93kD member of the CTX (Cortical Thymocyte Marker in Xenopus) subfamily of the Ig superfamily. It is a type I transmembrane protein with an N-terminal extracellular V-type and C2 type Ig domain separated by a J segment, a transmembrane domain and a cytoplasmic tail with a PDZ binding motif. JAM-4 is expressed in intestinal, renal, and mammary epithelial cells, spermatogonia, and hematopoietic stem cells. It is a cell adhesion molecule that mediates homophilic interaction. JAM-4 also interacts with the intracellular membrane-associated guanylate kinase inverted 1 (MAGI-1). An additional isoform containing a 100 residue insertion between Gln 125 and Asp 126 has been reported. Over the region used as immunogen, mouse JAM-4 shares 82% and 63% amino acid sequence identity with rat and human JAM-4, respectively.

Applications:
Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect recombinant mouse JAM4. The detection limit for rmJAM4 is approximately 5 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows less than 1% crossreactivity with rmJAM-A, rmJAM-B, and rmJAM-C.
Optimal dilutions should be determined by each laboratory for each application.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
50ug		-20°C	Blue Ice	Mouse	Sheep

Concentration:

As reported

Immunogen:

NS0-derived rmJAM4 (aa16-140)

Purity:

Purified by mouse JAM4 affinity chromatography and then biotinylated.

Form

Supplied as a lyophilized powder from a 0.2um filtered solution in PBS containing 50ug of BSA per 1ug of antibody. Reconstitute with sterile TBS, pH 7.3 (20mM Trizma base, 150mM sodium chloride) containing 0.1% BSA, 40-50% glycerol. If 1ml of buffer is us

Specificity:

Selected for use as a detection antibody in mouse JAM4 Western Blots.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.