Technical Data
J8010-01C
Junctional Adhesion Molecule 2 (JAM 2, JAM2, JAM-2, C21orf43, CD322, CD322 Antigen, JAM IT/VE JAM, Junction Adhesion Molecule B, JAM B, JAMB, JAM-B, PRO245, Vascular Endothelial Junction Associated Molecule, VE JAM, VEJAM, VE-JAM)
Description:
Junctional Adhesion Molecule 2 (JAM 2) is a novel junctional adhesion molecule and a member of the immunoglobulin superfamily molecules. The JAM 2 transcript is highly expressed during embryogenesis and is detected in lymph node and Peyer’s patches RNA of adult mice. JAM 2 participates in interendothelial junctional complexes.

Applications:
Suitable for use in Flow Cytometry, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10-1:25; 10ul labels 10e6 cells in 100ul
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from immunized Fischer rats.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG2a10B1409Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Recombinant soluble Junctional Adhesion Molecule 2 (JAM 2).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes mouse JAM 2. Species Crossreactivity: human. In adult murine tissue, expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, CD322 expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aurrand-Lions, M. et al. (2001) Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood. 98: 3699 - 3707, 2. Johnson-Léger, C.A. et al. (2002) Junctional adhesion molecule - 2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 100: 2479 - 2486, 3. Aurrand-Lions, M. et al. (2001) JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J. Biol. Chem. 276: 2733 - 2741.