		Technical Data
J8025-01	Junctional Adhesion Molecule C (JAM-C)

Description:
Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Applications:
Can be used for Immunoprecipiation, immunoassays as detector antibody, Immunohistology on frozen sections, Flow Cytometry and ELISA. Also useful for inhibition of biological activity.

Recommended Dilution:
Immunohistology: 1:10
Flow Cytometry: 1:10
ELISA: 1:10
For inhibition of biological activity dilutions have to be made according to the amounts of JAM-C to be inhibited.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	6A591	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Mouse	Rat

Concentration:

~0.1mg/ml

Immunogen:

Recombinant, soluble JAM-2

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, containing 0.1% BSA.

Specificity:

Recognizes mouse JAM-C. Also reacts with human JAM-C.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Aurrand-Lions, M et al; JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J Biol Chem 2001, 276: 2733. 2. Aurrand-Lions, M et al; Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood 2001, 98: 3699. 3. Johnson-Leger, C et al; Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration. Blood 2002, 100: 2479.