Technical Data
J8025-01
Junctional Adhesion Molecule C (JAM-C)
Description:
Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Applications:
Can be used for Immunoprecipiation, immunoassays as detector antibody, Immunohistology on frozen sections, Flow Cytometry and ELISA. Also useful for inhibition of biological activity.

Recommended Dilution:
Immunohistology: 1:10
Flow Cytometry: 1:10
ELISA: 1:10
For inhibition of biological activity dilutions have to be made according to the amounts of JAM-C to be inhibited.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6A591Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant, soluble JAM-2
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.1% BSA.
Specificity:
Recognizes mouse JAM-C. Also reacts with human JAM-C.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aurrand-Lions, M et al; JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J Biol Chem 2001, 276: 2733. 2. Aurrand-Lions, M et al; Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood 2001, 98: 3699. 3. Johnson-Leger, C et al; Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration. Blood 2002, 100: 2479.