Technical Data
J8025-01
Junctional Adhesion Molecule C (JAM-C, JAM-2)
Description:
Junctional adhesion molecule-C (JAM-C) also known as JAM-2 is a 45kD cell adhesion molecule (CAM). JAM-C is a transmembrane protein which is a member of the immunoglobulin superfamily found at intercellular junctions of endothelial cells. JAM-C belongs together with JAM-A (JAM or JAM-1) and JAM-B (VE-JAM or JAM-3) to a family of adhesion proteins with a V-C2 immunoglobulin domain organization. JAM plays an important role in tight junctions where it is involved in cell-to-cell adhesion through homophilic interaction. It codistributes with other tight junction components as ZO-1, 7H6 antigen, cingulin and occludin. JAM-C is potentially involved in the junctional sealing of the vascular endothelium, in particular of high endothelial venules (HEV). In adult murine tissue JAM-C expression is reported to be restricted to high endothelial venules of lymphoid organs, lymphoendothelial cells and endothelial cells in kidney. In humans, JAM-C expression is not restricted to endothelial cells, but is also expressed on human lymphocytes.

Applications:
Suitable for use in ELISA, Flow Cytometry, Immunohistochemistry (frozen) and Immunoprecipitation. Also useful for inhibition of biological activity. Not suitable for use in WB and Immuohistochemistry on paraffin sections. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (frozen): 1:50
Flow Cytometry: 1:50
For inhibition of biological activity dilutions have to be made according to the amounts of JAM-C to be inhibited.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2aCRAM-18 F26Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Recombinant soluble JAM-C (UniProt Accession #Q9D8B7)
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, containing 0.1% BSA.
Specificity:
Recognizes mouse JAM-C. Species Crossreactivity: human
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aurrand-Lions, M et al; JAM-2, a novel immunoglobulin superfamily molecule, expressed by endothelial and lymphatic cells. J Biol Chem 2001, 276: 2733. 2. Aurrand-Lions, M et al; Heterogeneity of endothelial junctions is reflected by differential expression and specific subcellular localization of the three JAM family members. Blood 2001, 98: 3699. 3. Johnson-Leger, C et al; Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration. Blood 2002, 100: 2479.