Technical Data
K0005-09B
Kallikrein 6 (KLK6) (Neurosin)
Description:
Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes. Neurosin (KLK6), one of these, is atrypsin-like protease dominantly expressed in the human brain but also in a variety of other tissues. The human tissue kallikrein gene family contains 15 members that play important roles in cancer. Notably, KLK1, also known as tissue kallikrein, cleaves kininogen to release the vasoactive kinin peptide, bradykinin or lysyl-bradykinin. KLK3, called prostate specific antigen (PSA), is an established tumor marker that aids in the diagnosis, staging, and follow up of prostate cancer. KLK4 is specifically expressed in the prostate and overexpressed in prostate cancer. KLK5 is widely expressed but found at high levels in skin, breast, brain and testis; overexpression is an indicator of poor prognosis in ovarian cancer. KLK8 is expressed in the brain and is a novel marker of ovarian and cervical cancer.

Applications:
Suitable for use in ELISA and Western Blot

Recommended Dilutions:
ELISA: 1:1000

Hybridoma:
Hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Neurosin protein.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG18G2Affinity Purified
SizeStorageShippingSourceHost
50ul-20CBlue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Recombinant human Neurosin (23-240aa) purified from E. coli
Purity:
Purified by protein-G affinity chromatography.
Form
Supplied as a liquid In PBS, pH 7.4, with 0.1% Sodium Azide.
Specificity:
Recognizes human Kallikrein 6 (KLK6) (Neurosin)
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Luo LY et al.,(2006) Tumour Biol 27(5):227234
Pedro FA et al.,(2006) J Biol Chem 281(6):31163126
Pampalakis G et al.,(2006) Biol Chem 387(6):773782