Technical Data
K0005-14C
Kallikrein 11 (KLK11, Hippostasin, Trypsin-like serine protease and PRSS20)
Description:
Kallikrein 11 is expressed in brain, skin, ovary and prostate. Increased levels of Kallikrein 11 have been reported in prostate and ovarian carcinoma. It is a serine protease. Kallikrein 11 (KLK11), also known as hippostasin, trypsin-like serine protease and PRSS20, is a member of human tissue kallikrein family.6 Two alternatively spliced forms exist, resulting in 250 (isoform 1) and 282 (isoform 2) amino acid sequences, respectively.1-4. Isoform 1 consists of a signal peptide (residues 1-18), a short pro peptide (residues 19-21) and the mature chain
(residues 22-250). Isoform 2 is identical to isoform 1, except that a 32 amino acid segment is inserted in isoform 2 before residue1 in isoform 1. Isoform 1 is predominantly expressed in brain whereas isoform 2 is preferentially expressed in prostate. KLK11 is a novel marker for ovarian and prostate cancer carcinomas.7-9 rhKLK11, after being activated by thermolysin, is active against a thioester substrate.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Direct ELISA: 0.5-1.0ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is ~5ng/well.

Western blot: 1-2ug/ml with the appropriate secondary reagents to detect human Kallikrein 11. The detection limit for rhKallikrein 11 is approximately 5ng/lane under
non-reducing conditions.

Optimal dilutions to be determined by the researcher.

Recommended Positive Control Tissue:
Ovarian tumor

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG3Affinity Purified
SizeStorageShippingSourceHost
500ug4C Do Not FreezeBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived, purified recombinant, human Kallikrein 11, aa 22-250
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in 0.2um filtered solution in PBS with 5% trehalose.
Specificity:
Recognizes human Kallikrein 11 in direct ELISA and Western Blot. Less than 5% crossreactivity was observed with rhKLK3 and rhKLK5.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yoshida, S. et al., 1998, Biochim. Biophys. Acta 1399:225 - 228.
2. Yousef, G.M. et al., 2000,Genomics 63:88 - 96.
3. Mitsui, S. et al., 2000, Biochem. Biophys. Res. Commun. 272:205 - 211.
4. Gan, L. et al., 2000, Gene 257:119 - 130.
5. Edwards, K.M. et al., 1999, J. Biol. Chem. 274:30486 - 30473.
6. Yousef, G.M. and E.P. Diamandis, 2001, Endocrine Rev. 22:184 - 204.
7. Diamandis, E.P. et al., 2002, Cancer Res. 62:295 - 300.
8. Nakamura, T. et al., 2003, Urology 61:1042 - 1046.
9. Borgono, C.A. et al., 2003, Int. J. Cancer 106:605 - 610.