Technical Data
K0015
Kanamycin Sulfate 50mg/ml Solution
5x20ml
10x20ml
Biochemicals Storage: 4CShipping: Blue Ice
Kanamycin sulfate is an antibiotic that belongs to the group of drugs called aminoglycosides. It binds to ribosomal components and inhibits protein synthesis. Kanamycin sulfate appears to prevent bacteria from making their cell walls; thus the cells die. It is used to treat many sensitive gram-negative, some gram-positive bacteria and and mycoplasmas in cell culture. Kanamycin is inactivated by an aminoglycoside phospho transferase (MW 25,000) in the periplasmic space.

Application:
Suitable for use as a selectable marker for cosmid vectors used in transfection of mammalian cells.

Form: Filter sterilized (0.2um) solution in 0.9% saline. Supplied in 20ml amber screw-cap vials.
pH:6.5-8.5
Appearance: Clear solution
Chromatographic Purity: To pass USP
Identity: To pass USP
Activity: 750ug/mg
Crystallinity: To pass USP
Specific Rotation: +107 to +121
Endotoxin: 2Eu/mg
Kanamycin B Content (TLC): 4%
pH (Acidity or Alkalinity): 6.5-8.5
Sulfate: 15.0-17.0%
Sulfated Ash: 0.5%
Abnormal Toxicity: To pass BP98
Depressor Substance: To pass BP98

Working Concentration:
Cell/Tissue Culture: ~30ug/ml
Stringent Plasmids: 10ug/ml
Relaxed Plasmids: 50ug/ml

Storage and Stability:
Store liquid at 4C. Stable for for 12 months at 4C. Further dilutions can be made in assay buffer. Stability at 37C: 5 days.

CAS Number:
25389-94-0

Molecular Formula:
C18H36N4O11H2SO4

Molecular Weight:
582.58
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.