Technical Data
K0080
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Immunohistochemical detection of immunoglobulins is useful in determining the lymphoid origin of tissues and in the assessment of lymphoid proliferations as reactive or malignant. The assessment of light chain status is particularly important in this regard as it may be used to demonstrate the clonal nature of a tumor.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (frozen and formalin/paraffin): 1-2ug/ml. for 30min at RT.
Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.

Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Cellular Localization:
Cytoplasmic

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.343Affinity Purified
SizeStorageShippingSourceHost
500ul4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
0.2mg/ml
Immunogen:
B lymphoma cells.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide. See K0080X
Specificity:
Recognizes the kappa light chain of immunoglobulin. Species Crossreactivity: Human. Does not react with Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Korkolopoulou P, et. al. Leukemia and Lymphoma, 1994, 13(1-2):151-9. 2. Abe M, et al. American Journal of Clinical Pathology, 1993, 100(1):67-74. 3. Berg AM, et al. Annals of Otology, Rhinology and Laryngology, 1993, 102(11):884-9. 4. Takahashi H, et al. Pathology, Research and Practice, 1993, 189:300-11. 5. Momose H, et al. Human Pathology, 1992, 23(10):1115-9. 6. Takahashi H, et al. Journal of Oral Pathology and Medicine, 1992, 21(7):318-25. 7. Axelrod O, et al. Blood, 1991, 77(7):1484-90. 8. Chen YT, et al. Human Pathology, 1991, 22(12):1249-57. 9. Faa G, et al. Journal of Hepatology, 1991, 12(1):75-82. 10. Farhi DC and Luckey CN. American Journal of Clinical Pathology, 1991, 95(5):702-8.