Technical Data
K0080X
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (BSA & Azide Free)
Description:
Reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (frozen and formalin/paraffin): 1:400-1:800 for 30 min at RT.
Note: Staining of formalin/paraffin tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k0.N.343Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
B lymphoma cells. Cellular Localization: Cytoplasmic. MW of antigen: 25kD
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS. Also available with BSA and azide. See K0080.
Specificity:
Highly specific to the kappa light chain of immunoglobulin. Species Crossreactivity: Human. Does not react with Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Korkolopoulou P, et al., Leukemia and Lymphoma, 1994, 13(1-2):151-9. 2. Abe M;, et al., American Journal of Clinical Pathology, 1993, 100(1):67-74. 3. Berg AM, et al., Annals of Otology, Rhinology and Laryngology, 1993, 102(11):884-9. 4. Takahashi H, et al., Pathology, Research and Practice, 1993, 189:300-11. 5. Momose H, et al., Human Pathology, 1992, 23(10):1115-9. 6. Takahashi H, et al., Journal of Oral Pathology and Medicine, 1992, 21(7):318-25. 7. Axelrod O, et al., Blood, 1991, 77(7):1484-90. 8. Chen YT, et al., Human Pathology, 1991, 22(12):1249-57. 9. Faa G, et al., Journal of Hepatology, 1991, 12(1):75-82. 10. Farhi DC, et al., American Journal of Clinical Pathology, 1991, 95(5):702-8.