Technical Data
K0090-04
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

IgG Goat anti mouse IgG: RPE (rat adsorbed)

Fusion Partners: Spleen cells from immunized BALB/c mice fused with cells of the mouse NS1 myeloma cell line.

Applications:
Flow Cytometry: 1:100
Immunohistochemistry (Frozen)
Optimal working dilutions to be determined by researcher.

Flow Cytometry: Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
TypeIsotypeCloneGrade
MabIgG2a3H2575Affinity Purified
SizeStorageShippingSourceHost
250ug-20°CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
F(ab')2 fragment of rat IgG.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Recognizes a determinant on rat kappa chains and shows a marked preference for binding to the Igk-a allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG strains but not in ACI, DA, F344, MSU or SHR.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hunt, S.V. & Fowler, M.H. (1981) Cell & Tissue Kinetics 14: 445-464.2. Gasser, D.L. (1977) Advances in Immunology 25: 93-139.3. Barclay, A.N. (1981). Immunology 42: 593-600.