Technical Data
K0090-06
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (Biotin)
Description:
Useful in the identification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

Applications:
Suitable for use in ELISA and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: Neat, Use 10ul of the suggested working dilution to label 10e6 cells in 100ul.
Optimal working dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized PVG RT1c rats were fused with cells of the rat Y3.Ag.1.2.3 myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

TypeIsotypeCloneGrade
MabIgG2b3H2573Highly Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceMouseRat
Concentration:
~0.1mg/ml
Immunogen:
Purity:
Purified by Ion Exchange chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, and 0.09% sodium azide. Labeled with Biotin.
Specificity:
mouse kappa immunoglobulin light chains. No reactivity seen against mouse lambda light chains in ELISA assays.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Galfre, G. et al., 1979, 277; Nature: 131-133.