Technical Data
K0090-15
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809)
Description:
Useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas. The most common feature of these malignancies is the restricted expression of a single light chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant. Immunoglobulin bearing Kappa light chains are present on about 60% of normal B lymphocytes.

Applications:
Suitable for use in indirect immunofluorescence and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Indirect Immunofluorescence/FC: 1ug/1x10e6 cells or 100ul peripheral blood.
Optimal dilution to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15F66Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
Kappa Light Chain
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide before the addition of glycerol to 40%.
Specificity:
Recognizes Kappa light chains of human immunoglobulin and does not react with Lambda light chain or heavy chain. Reacts with CD19 positive lymphocyte (54%) and is negative in other peripheral blood cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Tadamitsu K, K. Hitoshi, A.E.G.Kr. van dem Borne, et al., eds. 1997. Leukocyte Typing VI: White Cell Differentiation Antigens. P 210 240 Garland Publishing, Inc., New York.