Technical Data
K0100-01A
Kappa Light Chain (HCAK1, Ig kappa Chain C Region, IGKC, Immunoglobulin kappa Constant Region, Immunoglobulin kappa Light Chain, Kappa 1 Immunoglobulin Light Chain, Km, MGC111575, MGC62011, MGC72072, MGC88770, MGC88771, MGC88809) (HRP)
Description:
Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: 500-1000ng/ml
Immunohistochemistry: Frozen sections
Optimal working dilutions to be determined by researcher.

Recommended Pair:
Capture: I1904-84P
Detection: K0100-01A

Hybridoma:
Spleen cells from immunized LOU/c rats were fused with cells of the rat IR983F myeloma cell line.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Labeled with horseradish peroxidase (HRP). Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. HRP conjugates are sensitive to light.
TypeIsotypeCloneGrade
MabIgG2a3H2577Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Purified mouse IgG1 kappa
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% thimerosal, 50% glycerol. Labeled with Horseradish Peroxidase (HRP).
Specificity:
Recognizes mouse kappa immunoglobulin light chain and does not crossreact with other mouse immunoglobulin classes or subclasses.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Delauney, T., et al., Rat (and mouse) monoclonal antibodies. III. ELISA measurement of Ig production in mouse hybridoma culture supernatants. J. Immunol. Methods. 131: 33-39 (1990). 2. Savignac. M. et al. (2010) Increased B cell proliferation and reduced Ig production in DREAM transgenic mice.
J Immunol. 185:7527-36.