		Technical Data
K0199-06X	Keratin 6 (BSA & Azide Free)

Description:
Keratins 6 and 16 are expressed in keratinocytes. These cells undergo rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK16 is strongly expressed in ~75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Applications:
Suitable for use in Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG2a	0.N.347	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to GSSTI-KYTTTS from the C-terminus of human keratin 6. Cellular Localization: Cytoplasmic.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Available with BSA and azide. See K0199-06.

Specificity:

Recognizes human cytokeratin 6 (CK6) at ~56kD. Species Crossreactivity: mouse.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Wetzels, R.H., et. al., American Journal of Pathology 138(3): 751-63 (1991). 2. Wetzels, R.H., Kuijpers, H.J., Lane, E.B., Leigh, I.M., Troyanovsky, S.M., Holland, R., van Haelst, U.J., Ramaekers, F.C., Basal cell-specific and hyperproliferation-related keratins in human breast cancer. American Journal of Pathology 138(3): 751-63 (1991).