Technical Data
K0199-06X
Keratin 6 (BSA & Azide Free)
Description:
Keratins 6 and 16 are expressed in keratinocytes. These cells undergo rapid turnover in the suprabasal region (also known as hyperproliferation-related keratins). Keratin 6 is found in hair follicles, suprabasal cells of a variety of internal stratified epithelia, in epidermis, in both normal and hyperproliferative situations. Epidermal injury results in activation of keratinocytes, which express CK6 and CK16. CK16 is strongly expressed in ~75% of head and neck squamous cell carcinomas. Expression of CK6 is particularly associated with differentiation.

Applications:
Suitable for use in Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a0.N.347Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to GSSTI-KYTTTS from the C-terminus of human keratin 6. Cellular Localization: Cytoplasmic.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins and no preservatives added. Available with BSA and azide. See K0199-06.
Specificity:
Recognizes human cytokeratin 6 (CK6) at ~56kD. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wetzels, R.H., et. al., American Journal of Pathology 138(3): 751-63 (1991). 2. Wetzels, R.H., Kuijpers, H.J., Lane, E.B., Leigh, I.M., Troyanovsky, S.M., Holland, R., van Haelst, U.J., Ramaekers, F.C., Basal cell-specific and hyperproliferation-related keratins in human breast cancer. American Journal of Pathology 138(3): 751-63 (1991).