Technical Data
K1700-10A
Ki-67 (Antigen Identified by Monoclonal Antibody Ki-67, KIA, MKI67, Proliferation Related Ki-67 Antigen)
Description:
Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumors is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Acetone and formalin fixed, paraffin-embedded sections): Neat. Incubate 60 minutes at RT. Paraffin requires high temperature unmasking.
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil or breast carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8L521Supernatant
SizeStorageShippingSourceHost
6ml-20°CBlue IceHumanMouse
Concentration:
Not Determined
Immunogen:
Human recombinant Ki67 protein containing the C-terminal 340aa. Cellular Localization: Nuclear.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, sodium azide, Proclin 300.
Specificity:
Recognizes human Ki-67 nuclear antigen of 345 and 395kD. It recognizes the native as well as recombinant fragments of Ki-67. Ki-67 nuclear antigen is associated with cell proliferation and is found throughout the cell cycle.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Key G et al. New Ki-67-equivalent murine monoclonal antibodies (MIB 1-3) generated against bacterially expressed parts of the Ki-67 cDNA containing three 62 base pair repetitive elements encoding for the Ki-67