Technical Data
K1759-24
KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)
Description:
KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor.

Applications:
Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Peptide ELISA: 1:1000
Western Blot: Detects a band at ~120kD in human kidney lysates
Immunohistochemistry (Paraffin; human kidney): 1-2ug/ml. Microwaved antigen retrieval with Tris/EDTA, pH 9. Shows perinuclear labeling of some glomerular cells and cytoplasmic staining of renal tubules.
Immunohistochemistry (paraffin; human uterus): 2-3ug/ml. Steamed antigen retrieval with citrate buffer, pH 6. Shows strong staining of the epithelium on endometrial glands.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa 951-963, QPVAVRGGGGKQV, from the C-terminus of human KIF5B (NP_004512). Cellular Localization: Uniformly distributed between the cell body and the processes in the neurons.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Recognizes human KIF5B at ~120kD. Species Crossreactivity: canine
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Niclas J et al. Cloning and localization of a conventional kinesin motor expressed exclusively in neurons. Neuron 12:1059-72 (1994).