Technical Data
K1759-24
KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)
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Technical Data |
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K1759-24 |
KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC) |
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Description: KIF5B is a kinesin. These are microtubule based motor proteins involved in the transport of organelles in eukaryotic cells. They typically consist of 2 identical, approximately 110 to 120kD heavy chains and 2 identical, approximately 60 to 70kD light chains. The heavy chain contains 3 domains: a globular N terminal motor domain, which converts the chemical energy of ATP into a motile force along microtubules in 1 fixed direction; a central alpha helical rod domain, which enables the 2 heavy chains to dimerize; and a globular C terminal domain, which interacts with light chains and possibly an organelle receptor. Applications: Suitable for use in Peptide ELISA, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Peptide ELISA: 1:1000 Western Blot: Detects a band at ~120kD in human kidney lysates Immunohistochemistry (Paraffin; human kidney): 1-2ug/ml. Microwaved antigen retrieval with Tris/EDTA, pH 9. Shows perinuclear labeling of some glomerular cells and cytoplasmic staining of renal tubules. Immunohistochemistry (paraffin; human uterus): 2-3ug/ml. Steamed antigen retrieval with citrate buffer, pH 6. Shows strong staining of the epithelium on endometrial glands. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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