Technical Data
K1759-24D
KIF5B (Kinesin Family Member 5B, Kinesin 5B, Kinesin 1, Kinesin Heavy Chain, KINH, KNS1, Ubiquitous Kinesin Heavy Chain, UKHC)
Description:
Kinesin belongs to the group of motor proteins known to convert chemical energy released from ATP into mechanical energy. Conventional kinesin, member of the kinesin superfamily comprising more than 100 proteins, is involved in the anterograde vesicle transport inneuronal cells. Kinesin purified from mammalian brain homogenates is a heterotetramer consisting of two heavy (120 to 130kD) and two light chains (60 to 70kD), resulting in a molecular mass about 400kD. Each heavy chain contains an N terminal globular motordomain with both a microtubule binding site and an ATPase active center, stalk region responsible for heavy chain dimerization and finally C terminal globular tail domain, which is implicated in cargo binding. Light chains may have a regulatory function.

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Stalk domain of human kinesin (amino acids 331-906) expressed in E. coli (FKHC3).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes the total level of human endogenous kinesin protein. Species Crossreactivity: mouse and porcine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Malcova-Janatova I, Richterova V, Draber P, Hasek J. (2004) Folia Microbiol (Praha) 49(6):665-70. (Unpublished)