Technical Data
The Wnt family of secreted glycoproteins mediate cell-cell interaction during cell growth and differentiation in both embryos and adults. (1,2) Canonical Wnt signaling by way of the b-catenin pathway is transduced by two receptor families, frizzled proteins and lipoprotein-receptor-related proteins 5 and 6 (LRP5/6) which binds Wnts and transmit their signal by stabilizing intracellular b-catenin.(3-6) Kremen1 and Kremen2 are high-affinity Dkk1 receptors that functionally cooperate with the secreted protein Dickkopf1 (Dkk1) to block Wnt/b-catenin signaling.(7) Kremen1 and Kremen2 are components of a membrane complex modulating canonical Wnt signaling through LRP6 in vertebrates.(7) Dkk1 has been shown to inhibit Wnt signaling by binding to and antagonizing LRP5/6 (8-10).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-2ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

PabIgGAffinity Purified
50ug4C (-20C Glycerol)Blue IceHumanRabbit
Synthetic peptide derived from the internal region of human, mouse and rat Kremen2 proteins.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide, before the addition of glycerol to 40%.
Recognizes human, mouse and rat Kremen2 proteins at ~51kD. No cross-reactivity with the related Kremen1 protein. Reactivity has been confirmed with myc-tagged mouse Kremen2-transfected 293T cell lysates. Species sequence homology: human and rat-100%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miller JR, et al. Oncogene 18:7860-7872, 1999.
2. Wodarz A and Nusse R. Annu Rev Cell Dev Biol 14:59-88, 1998.
3. Bhanot P, et al. Nature 382:225-230, 1996
4. Pinson KI, et al. Nature 407:535-538, 2000.
5. Tamai K, et al. Nature 407:530-535, 2000.
6. Wehrli M, et al. Nature 407:527-530, 2000.
7. Mao B, et al. Nature 417:664-667, 2002
8. Bafico A, et al. Nat Cell Biol 3:683-686, 2001.
9. Mao B, et al. Nature 411:321-325, 2001.
10. Semenov MV, et al. Curr Biol 11:951-961, 2001.