Technical Data
K9001-50
Kv1.3 (Kcna3, Charybdotoxin-sensitive Voltage-dependent Potassium Channel, HLK3)
Description:
Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:200 Rat brain membranes
Immunohistochemistry: Rat brain sections
Optimal dilutions to be determined by the researcher.

Control Antigen:
K9001-50A Supplied as a lyophilized powder. Reconstitute with 100ul of PBS, pH 7.4.
Western Blot Positive Control: 20ng per minigel lane.
Western Blot Negative Control: Preincubate 3ug of fusion protein

Storage and Stability:
Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer plus carrier protein (1% BSA).
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
~0.3mg/ml
Immunogen:
Synthetic peptide corresponding to aa523-575 of human Kv1.3 (Accession P22001). Epitope location: Intracellular, C-terminus.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 1% BSA, 5% sucrose, 0.025% sodium azide. Reconstitute with 50ul sterile 40-50% glycerol, ddH2O.
Specificity:
Recognizes human Kv1.3. Species Crossreactivity: rat, mouse. Does not recognize any other potassium channel antigens tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yuan, X.J. et al. (1998) Am. J. Physiol. 274, L621. 2. Greffrath, W. et al. (1998) J. Physiol. 513.2, 493.
3. Xu, C. et al. (1999) Am. J. Physiol.277, G1055. 4. Chung, Y.H. et al. (2000) Brain Res. 875, 164.
5. Desai, R. et al. (2000) J. Biol. Chem. 51, 39954.