Technical Data
Media
Lactobacilli MRS Agar (Powder)
L1021
Microbiological Grade
Storage 4°C    Shipping RT
Formulation per Liter: g/Liter
Animal Tissue, Enzymatic Digest10.0
Beef Extract10.0
Dextrose20.0
Sodium Acetate5.0
Yeast Extract5.0
Ammonium2.0
Potassium Phosphate Dibasic2.0
Tween 801.0
Magnesium Sulfate•7H2O0.1
Mangenese Sulfate•5H2O0.05
Agar, Bacteriological15.0
Total:70.0g/L
Suitable for use in the enumeration of Lactobacilli from food materials. Also used for the cultivation of Aerococcus viridans, Bifidobacterium coryneforme, Lactococcus plantarum, Leuconostoc sp., Pectinatus cerevisiiphilus, Pediococcus sp. and Sporolactobacillus inulinus. based on the formulations of deMan, Rogosa and Sharpe (MRS).


Directions per Liter Dissolve 70 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121ºC (15psi).

Storage and Stability: Store powdered media at 4ºC. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Microbiological Analysis (Growth): Lactogacillus casei Lactogacillus fermentum Lactogacillus plantarum

pH: 6.5 ± 0.2 at 25°C

Appearance: Homogeneous, tan powder

Solubility: Clear, light tan and complete

Certificate of Origin: Animal Tissue


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.