Technical Data
L1185-05
Lambda Light Chain
Description:
Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunohistochemistry (frozen & formalin/paraffin): 1-2ug/ml for 30 mins. at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 min., followed by cooling at RT for 20 min.
Optimal dilutions to be determined by the researcher.

Cellular Localization: Cytoplasmic.

MW of Antigen: 25kD.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a,k0.N.379Highly Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified human IgG myeloma proteins covalently coupled to polyaminostyrene micro beads.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 15mM sodium azide. Also available without BSA and azide. See L1185-05X.
Specificity:
Recognizes the lambda light chains of human immunoglobulins. Also recognizes free and Bence-Jones lambda light chains. Species Reactivity: Human. Does not react with Rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reimer CB et. al. Hybridoma, 1984, 3:263-75.