Technical Data
L1185-11D
Lambda Light Chain
Description:
Applications:
Suitable for use in Flow Cytometry and ELISA. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:5-1:10. Use 10ul to label 10e6 cells or 100ul washed whole blood.
Optimal working dilutions to be determined by researcher.

Hybridoma:
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line.

Recommended Negative Control:
I1904-78R: IgG1 Negative Control

Recommended Secondary Antibody:
I1904-66A: IgG, F(ab)2 (FITC) Pab Rb xMo

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H2592Affinity Purified
SizeStorageShippingSourceHost
200ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
~0.25mg/ml
Immunogen:
Purified human IgG.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, containing 0.09% sodium azide, before the addition of glycerol to 40%.
Specificity:
Recognizes human lambda light chains and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Reynolds, W M, et al. 1992. A simple technique for the determination of kappa and lambda immunoglobulin light chain expression by B cells in whole blood. J. Imm. Meth. 151:123-129.