Technical Data
L1225-40
Laminin A
Description:
Laminin is a protein found in the extracellular matrix, the sheets of protein that form the substrate of all internal organs also called the basement membrane. It is composed of one A chain (400kD) one B1 chain (215kD) and one B2 chain (205kD) all held together by disulfide bonds. The molecule has a cross like form with globular units near the ends of each chain, the sites where it is bound to Collagen IV, heparan sulfate, proteoglycan as well as to the surface of epithelial cells.

Applications:
Suitable for use in ELISA, RIA, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Suitable for use on paraffin embedded EHS tumor, or tissue frozen in OCT at-70C and treated with hyaluronidase.
Optimal dilution determined by the researcher.

Source:
Ascites

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2Q599Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseRat
Concentration:
~1mg/ml
Immunogen:
Laminin isolated from mouse EHS (Engelbreth-Holm-Swarm) tumor.
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in PBS, pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes EHS (Engelbreth-Holm-Swarm) mouse laminin, A chain. Recognizes the carboxy terminal globular domain on the A chain.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Skubitz, A.P. et al., J. Biol. Chem. (1988) 263: 4861-4868. 2. Schuger, L. et al., Dev. Biol. (1991) 146: 531-541. 3. Schuger, L. et al., Dev. Dynamics (1992) 195: 43-54. 4. Furtado, G.C. et al., Infect. Immun. (1992) 60: 4925-4931.