Technical Data
L1390
LAT (Linker for activation of T Cells)
Description:
Lat is an adapter protein expressed in lymphoid cells, which is a substrate for ZAP70 and Syk protein kinases. Phosphorylated LAT associates with GRB2, PLC-gamma1 and P85 PI 3-K. Expression of a mutant LAT protein missing tyrosine phosphorylation sites leads to inhibition of T-cell activation, suggesting that the protein is an important signal transducer in T-cells

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilution:
Western Blot: 2-4ug/ml detects LAT in RIPA lysates from jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transfered to nitrocellulose and probed with L1390 (0.6ug/ml). Proteins were visualized using goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence.
Immunoprecipitation: 8-10ug immunoprecipitated LAT from 500ug of Jurkat RIPA lysate
Optimal dilutions to be determined by the researcher.

Positive Antigen Control:
J9000 Jurkat lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
GST fusion protein corresponding to residues 31-233 of human LAT.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.01M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide. before the addition of glycerol to 30%.
Specificity:
Specific for both forms of human LAT (36 and 38kD). Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Zhang, W., et al., Cell. 92: 8392, 1998. Wange, R.I., et al., J. Biol. Chem. 270: 1873018733, 1995.