Technical Data
LAT (Linker for activation of T Cells)
Lat is an adapter protein expressed in lymphoid cells, which is a substrate for ZAP70 and Syk protein kinases. Phosphorylated LAT associates with GRB2, PLC-gamma1 and P85 PI 3-K. Expression of a mutant LAT protein missing tyrosine phosphorylation sites leads to inhibition of T-cell activation, suggesting that the protein is an important signal transducer in T-cells

Suitable for use in Western Blot and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 2-4ug/ml detects LAT in RIPA lysates from Jurkat cells. Jurkat cell lysate was resolved by electrophoresis, transfered to PVDF and probed with L1390 (2ug/ml). Proteins were visualized using IgG (HRP)goat anti-rabbit secondary antibody and a chemiluminescence detection system.
Immunoprecipitation: 8-10ug immunoprecipitated LAT from 500ug of Jurkat RIPA lysate
Optimal dilutions to be determined by the researcher.

Included Positive Antigen Control:
J9000: Jurkat lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
400ug-20CBlue IceHumanRabbit
GST fusion protein corresponding to residues 31-233 of human LAT.
Purified by Protein A affinity chromatography
Supplied as a liquid in Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide
Recognizes both forms of human LAT (36 and 38kD). Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang, W., et al., Cell. 92: 8392, 1998. 2. Wange, R.I., et al., J. Biol. Chem. 270: 1873018733, 1995.