Technical Data
LAT (36kD Phospho-tyrosine Adapter Protein, LAT1, Linker for Activation of T-cells Family Member 1, p36-38, pp36)
LAT is a transmembrane molecule that mediates signaling following the engagement of a number of cell surface receptors including TCR during mature T cell activation, pre-TCR during T cell development, Fc RIII on NK cells, and FceRI on mast cells. LAT is multiply phosphorylated on tyrosines by ZAP-70 and Syk, which leads to downstream effects that include release of intracellular calcium, PKC activation, MAPK activation and cytoskeletal reorganization. Palmitoylation of LAT is required for its localization to lipid rafts and efficient phosphorylation.

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG1,k11B.12Affinity Purified
25ug-20CBlue IceMouse
GST fusion protein corresponding to aa31-233 of human LAT, cytoplasmic domain.
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Recognizes human LAT. Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhang W et al. 1998. Cell 92:83. 2. Weber JR. et al. 1998. J. Exp. Med. 187:1157.