Technical Data
L1390-05A
LAT (LAT, linker for activation of T cells, LAT1, pp36, 36kD phospho-tyrosine adaptor protein)
Description:
Alternate Name: LAT, linker for activation of T cells, LAT1, pp36, 36 kD phospho-tyrosine adaptor protein

Domain Information:

Gene Ontology Terms: SH3/SH2 adaptor protein activity; immune response; integral to membrane; signal transduction

Application(s):
Peptide ELISA: antibody detection limit dilution 1:2,000. Western Blot: Approx 35kD band observed in Human PBMC lysates (predicted MW of 30kD according to NP_055202.1). Recommended for use at 1-3ug/ml.

Staining (1ug/ml) of Human PBMC lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Peptide Blocking:
Corresponding peptide is available for Peptide Blocking studies. See L1390-05A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.
TypeIsotypeCloneGrade
PabAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
C-GAPDYENLQELN, C Terminus
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in Tris saline, pH 7.2, 0.5% BSA, 0.02% sodium azide.
Specificity:
This antibody is expected to recognize isoform a (NP_055202.1), isoform b (NP_001014987.1 and NP_001014989.1) and isoform c (NP_001014988.1). Species Crossreactivity: human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Finco TS, Kadlecek T, Zhang W, Samelson LE, Weiss A. , LAT is required for TCR-mediated activation of PLCgamma1 and the Ras pathway., Immunity. 1998 Nov;9(5):617-26.