Technical Data
Media
Lauryl Sulfate Broth w/MUG (Powder)
L1400-05
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/Liter
Formulation per Liter:
Pancreatic Digest of Casein20.0
Lactose5.0
Sodium Chloride5.0
Potassium Phosphate Dibasic2.75
Potassium Phosphate Moonobasic2.75
Sodium Lauryl Sulfate0.1
MUG0.05
Total per Liter35.56
500g makes 14Liters
Rapid detection of E. coli from water and food samples by fluorogenic detection methods.

Lauryl Sulfate Broth was formulated by Mallmann and Darby for detection of coliform bacteria in water. This is a selective medium because it contains sodium lauryl sulfate which inhibits Gram positive microorganisms. Lactose is added to detect rapid lactose fermentation by coliforms. The addition of 4- methylumbelliferyl-B-D-glucuronide (MUG) to Lauryl Sulfate Broth allows for the rapid detection (4-24 hours) of E. coli-When the incubated medium is observed for fluorescence using a long-wave UV light (365 nm) source. It can also detect anaerogenic E-coli strains. Most strains of E. coli produce B- glucuronidase which hydrolyzes MUG to the fluorogenic compound 4- methyl-umbelliferone. Studies by Feng and Hartman noted that most E coli strains (96%) and all enterotoxigenic E coli strains (100%) and a moderate number of Salmonella (17%) and Shigella (40%) stains produce B-glucuronidase. False positives can also be caused by Streptococcus sp.

Quality Control Summary:
Expected cultural response after 18-24 hours at 35.0C.

Organism Result
Escherichia coli ATCC 25922 Growth, Gas +, Fluorescence +
Proteus mirabilis ATCC 12453 Growth, Gas -, Fluorescence -
Staphylococcus aureus ATCC 25923 No Growth






Directions per Liter Dissolve 35.56 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage Store at 0-5C (store unopened media at 2-30C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Appearance: White to off-white crystalline powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Mallmann, W. L., and C. W. Darby. 1941. Uses of a lauryl sulphate tryptose broth for the detection of coliform organsisms. Am J. Public Health. 31:127.