Technical Data
Membrane Lauryl Sulfate Broth (Powder)
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/Liter
Formulation per Liter:
Peptic digest of animal tissue39.0
Yeast extract6.0
Sodium Lauryl Sulfate1.0
Phenol red0.2
Total per Liter35.51
500g makes 14Liters
Standard methods medium for growth of coliform bacteria in water and food.

Membrane Lauryl Sulfate Broth is used for determination of Escherichia coli and coliforms in water, using membrane filter technique.

Directions per Liter Dissolve 76.2 grams per liter of distilled/deionized (DDI) water, heating with stirring until completely solubilized, adjusting pH as necessary. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage Store at prepared media at 4C and protect from direct sunlight. (store unopened powder media at 2-25C). Opened bottles should be capped tightly and kept in a dark, low humidity environment.

Appearance: Red powder Solubility

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Mallmann, W. L., and C. W. Darby. 1941. Uses of a lauryl sulphate tryptose broth for the detection of coliform organsisms. Am J. Public Health. 31:127.