Technical Data
Lck Interacting Molecule (LIME, dJ583P15.4, FLJ20406, Lck Interacting Membrane Protein, Lck Interacting Transmembrane Adaptor 1, LIME1, LP8067, RP4 583P15.5)
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide ENVYESIKEMGL, representing the C-Terminus of the human protein (residues 258-269), according to NP_076173 (Mouse).
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH 7.2. 0.5% BSA, 0.02% sodium azide.
Species Crossreactivity: Reacts with Mouse. Predicted to react with Rat due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hur EM et al. LIME, a novel transmembrane adaptor protein, associates with p56lck and mediates T cell activation. J Exp Med 198:1463-73 (2003). PubMed: 14610044