		Technical Data
L1566-06	Lck Interacting Molecule (LIME, dJ583P15.4, FLJ20406, Lck Interacting Membrane Protein, Lck Interacting Transmembrane Adaptor 1, LIME1, LP8067, RP4 583P15.5)

Description:
LIME (Lck-interacting molecule) is a new raft-associated adaptor protein involved in regulation of T cell activation by coreceptors. LIME becomes tyrosine phosphorylated after cross-linking of the CD4 or CD8 coreceptors. Phospho-LIME associates with the Src family kinase Lck and its negative regulator, Csk. Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts, increased phosphorylation of Lck and higher Ca2+ response to CD3 stimulation.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2a	8L573	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

COOH-terminal peptide comprising residues 281-296 of the human LIME conjugated to keyhole limpet hemocyanin.

Purity:

Purified by Protein A affinity chromatography.

Form

15mM Sodium Azide in PBS. pH 7.4

Specificity:

Human Lck-interacting molecule (LIME) Species Crossreactivity: Crossreacts with Human.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Brdickova N et al. LIME: a new membrane Raft-associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J Exp Med 198:1453-62 (2003).