Technical Data
L1663-07
Legionella pneumophila
Description:
Legionella pneumophila is the causative agent of the Legionnaires' disease, a potential fatal pneumonia. It was isolated after an outbreak of pneumonia at an American legion convention in Philadelphia. The strain Philadelphia 1 is derived from the original 1976 isolate. Legionella pneumophila is found in biofilms and fresh and industrial water systems worldwide. It can survive and replicate intracellularly in free-living protozoa, often in aquatic biofilms.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin-fixed paraffin embedded sections
Immunofluorescence: 1:20 in PBS.
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C. Stable for 12 months after receipt at 4C. Do not freeze. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at 4C. Reconstituted product is stable for 12 months at 4C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG35F241Affinity Purified
SizeStorageShippingSourceHost
50ug4C Do Not FreezeBlue IceMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Legionella pneumophila
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes heat-resistant L. pneumophila serogroup 1-specific lipopolysaccharide antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sethi, K.K. & Brandis, H., Establishment of Hybridoma Cell Lines Secreting Anti-Legionella pneumophila Serogroup 1 Monoclonal Antibodies with Immunodiagnostic Potential. Zbl. Bakt. Hyg., Abt. Orig. A 255: 294-298 (1983).