Technical Data
L1663-07
Legionella pneumophila
Description:
Legionella pneumophila is the causative agent of the Legionnaires' disease, a potential fatal pneumonia. It was isolated after an outbreak of pneumonia at an American legion convention in Philadelphia. The strain Philadelphia 1 is derived from the original 1976 isolate. Legionella pneumophila is found in biofilms and fresh and industrial water systems worldwide. It can survive and replicate intracellularly in free-living protozoa, often in aquatic biofilms.

Applications:
Suitable for use in Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and paraffin sections
Immunofluorescence: 1:20 in PBS.
Optimal dilutions to be determined by researcher.

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG35F241Affinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceMouse
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Legionella pneumophila
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute with 1ml sterile ddH2O.
Specificity:
Recognizes heat-resistant L. pneumophila serogroup 1-specific lipopolysaccharide antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Sethi KK and H Brandis. Establishment of Hybridoma Cell Lines Secreting Anti-Legionella pneumophila Serogroup 1 Monoclonal Antibodies with Immunodiagnostic Potential. Zbl. Bakt. Hyg., Abt. Orig. A 255:294-298 (1983)