		Technical Data
L1669-01S	Leptin (Ob, Obese Protein, Obesity Factor)

Description:
Human OB, also named leptin, is a 16kD protein in human plasma. It is first identified by positional cloning. It is important in the regulation of body weight. It can lead to weight loss, decreased food intake, reduction of body fat. Also OB plays important roles in reproduction, immunological response and neuro-endocrine signaling.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
250ug		4°C (-20°C Glycerol)	Blue Ice	Human	Rabbit

Concentration:

~1mg/ml

Immunogen:

E. coli-expressed human OB.

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.1% sodium azide before the addition of glycerol to 40%.

Specificity:

Reacts with human OB.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Positional cloning of the mouse obese gene and its human homologue. Nature 1994, 372: 425-432 2. Weight-reducing effects of the plasma protein encoded by the obese gene. Science 1995, 269: 543-546 3. The biology of leptin: a review. J Anim Sci 1998, 76 (5): 1405-1420.