Technical Data
L1669-03
Leptin (Ob, Obese Protein, Obesity Factor)
Description:
Leptin (anti-obesity protein) is the product of the ob (obese) gene in mice and is involved in appetite control. The gene is expressed in adipose tissue and it is thought to be important in the regulation of fat stores. Mice of ob/ob genotype develop a form of diabetes similar to type II (non-insulin dependent) diabetes.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:4000
Immunohistochemistry (frozen sections): 1:1000
Immunocytochemistry: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ul4C (-20C Glycerol)Blue IceMouseRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa 25-44, QKVQDDTKTLIKTIVTRIND of mouse leptin.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS containing 1mg/ml BSA, 0.05% sodium azide.
Specificity:
Recognizes mouse leptin. Species Crossreactivity: human, rat and sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.