		Technical Data
L1670-12C	Leptin (LEP, Leptin Murine Obesity Homolog, Leptin Precursor Obesity Factor, Ob, Obese Protein, Obesity, Obesity Factor, Obesity Homolog Mouse, OBS)

Description:
Leptin, the product of the ob (obese) gene, is a 16kD protein consisting of 146 amino acid residues. Leptin is produced in the adipose tissue, and is considered to play an important role in appetite control, fat metabolism and regulation of body weight. It targets the central nervous system, particularly hypothalamus, affecting food intake. Leptin levels are high in most obese individuals. Studies have shown that it may also influence reproductive function.

Cellular Localization: Secreted

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	8L580

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

Recombinant full length protein (Human).

Purity:

As reported

Form

Supplied as a liquid in PBS, pH 7.2.

Specificity:

Recognizes recombinant human leptin and leptin circulating in human blood. Species Crossreactivity: Crossreacts with Human. Not yet tested in other species, but may be crossreactive to mouse and rat due to 85% and 84% homology to human leptin respectively.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

Rahmouni K & Haynes WG Leptin and the cardiovascular system. Recent Prog Horm Res 59:225-44 (2004). El-Atat F et al. Obesity and hypertension. Endocrinol Metab Clin North Am 32:823-54 (2003). Zhang Y et al. Positional cloning of the mouse obese gene and its human homologue. Nature 372:425-32 (1994).