Technical Data
Leukotriene A4 (LTA4) Hydrolase
Leukotriene A4 hydrolase (LTA4 hydrolase) has been cloned from a variety of species including human, rat, and guinea pig.1,2,3,4 These species exhibit ~90% homology at the amino acid level. Human LTA4 hydrolase has a calculated molecular mass of 69 kDa based on the deduced amino acid sequence.1,2

Suitable for use in Western Blot.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
Not determined
Recombinant human LTA4 hydrolase
Purified by immunoaffinity chromatography.
Supplied as a liquid in TBS, pH 7.4.
Recognizes Lhuman eukotriene A4 (LTA4) Hydrolase.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Funk, C.D., Rådmark, O., Fu, J.Y., et al. Molecular cloning and amino acid sequence of leukotriene A4 hydrolase. Proc. Natl. Acad. Sci.USA 84, 6677-6681 (1987).
2 Mancini, J.A. and Evans, J.F. Cloning and characterization of the human leukotriene A4 hydrolase gene. Eur. J. Biochem. 231, 65-71 (1995).
3. Minami, M., Ohno, S., Kawasaki, H., et al. Molecular cloning of a cDNA coding for human leukotriene A4 hydrolase. Complete primary structure of an enzyme involved in eicosanoid synthesis. J. Biol. Chem. 262, 13873-13876 (1987).
4. Makita, N., Funk, C.D., Imai, E., et al. Molecular cloning and functional expression of rat leukotriene A4 hydrolase using the polymerase chain reaction. FEBS Lett. 299, 273-277 (1992).
5. Minami, M., Mutoh, H., Ohishi, N., et al. Amino-acid sequence and tissue distribution of guinea-pig leukotriene A4 hydrolase. Gene 161, 249-251 (1995).