Leukotriene A4 hydrolase (LTA4 hydrolase) has been cloned from a variety of species including human, rat, and guinea pig.1,2,3,4 These species exhibit ~90% homology at the amino acid level. Human LTA4 hydrolase has a calculated molecular mass of 69 kDa based on the deduced amino acid sequence.1,2
Suitable for use in Western Blot.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Funk, C.D., Rådmark, O., Fu, J.Y., et al. Molecular cloning and amino acid sequence of leukotriene A4 hydrolase. Proc. Natl. Acad. Sci.USA 84, 6677-6681 (1987).|
2 Mancini, J.A. and Evans, J.F. Cloning and characterization of the human leukotriene A4 hydrolase gene. Eur. J. Biochem. 231, 65-71 (1995).
3. Minami, M., Ohno, S., Kawasaki, H., et al. Molecular cloning of a cDNA coding for human leukotriene A4 hydrolase. Complete primary structure of an enzyme involved in eicosanoid synthesis. J. Biol. Chem. 262, 13873-13876 (1987).
4. Makita, N., Funk, C.D., Imai, E., et al. Molecular cloning and functional expression of rat leukotriene A4 hydrolase using the polymerase chain reaction. FEBS Lett. 299, 273-277 (1992).
5. Minami, M., Mutoh, H., Ohishi, N., et al. Amino-acid sequence and tissue distribution of guinea-pig leukotriene A4 hydrolase. Gene 161, 249-251 (1995).