Technical Data
L2053-02A
Lewis A (Blood Group Lewis a, Lewis A Antigen)
Description:
Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin-type, and are detected on erythrocytes, certain epithelial cells and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter-related specificities belong to this group of antigens including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y and precursor type 1 chain antigens. Increased expression of Lewis B has been reported in gastric and pancreatic tumors as well as in colorectal cancer.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (paraffin): 5ug/ml. Use sodium citrate for heat-mediated antigen retrieval.
Immunohistochemistry (Frozen): 1:10-1:2000
ELISA: 1:2000
Western Blot: 1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
MabIgG18L586Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human tissue/cell preparation. Mucin isolated by size-exclusion chromatography from ovarian cyst fluid (pure endocervical type according to the Fenoglio’s classification).
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes human Lewis A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Creuzot-Garcher, C., et al., Invest. Ophthalmol. Vis. Sci. 40: 1631-1636 (1999). 2. Torrado, J., et al., Gastroenterology 102: 424-430 (1992). 3. Bara, J., et al., Biochem. J. 254: 185-193 (1988). 4. First International Workshop on Monoclonal Antibodies Against Red Blood Cell and Related Antigens. Vol II: Other blood group systems. (1987).