Technical Data
LHX2/Lim (Lim Region Containing Transcription Factor)
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: 1:200-1:1000
Optimal dilutions to be determined by the researcher.

Immunohistochemistry Protocol:
1. Dissect tissues. Freeze on dry ice.
2. Cut on a cryostat ~10um slices.
3. Dry slides at RT for 30-90 minutes.
4. Fix with cold Acetone/Methanol (50/50) for 2 min then either process further or store at -20C. Alternative is fixation with 4% PFA and treatment with a trypsin solution (0.05% trypsin, incubation for 10 min. at 37C, followed by 3 washes with PBS).
5. Let air dry.
6. Treat with PBS for 5 minutes.
7. Incubate in 50mM ammonium chloride for 30 minutes.
8. Treat with PBS for 5 minutes.
9. Incubate with Blocking Serum for 30-45 minutes.
10. Incubate with appropriately diluted L2075 for 90 minutes.
11. Wash 3 times for 5 minutes.
12. Incubate with fluorescent conjugated secondary anti-rabbit IgG for 30 minutes.
13. Wash 3 times for five minutes. Mount.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to aa239-254 af human LHX2/Lim2.
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. No preservative added.
Recognizes the human Lim homeobox containing transcription factor LHX2. No significant sequence homology with other proteins. Species Crossreactivity: rodent.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.