Technical Data
L2496-13
Lipase, Pancreatic (Pancreatic Triacylglycerol Lipase, Pancreatic Lipase, PL, PNLIP)
Description:
Pancreatic lipase (PNLIP) fulfills a key function in dietary fat absorption by hydrolyzing triglycerides into diglycerides and subsequently into monoglycerides and free fatty acids. By screening a human pancreas cDNA library with degenerate oligonucleotides designed from the amino acid sequences of human, canine, and porcine PNLIP, Giller et al. (1992) isolated cDNAs encoding 2 novel PNLIP-related proteins, PNLIPRP1 (604422) and PNLIPRP2, which they called PLRP1 and PLRP2, respectively. The predicted 469-amino acid PNLIPRP2 protein contains a 17-amino acid signal peptide and the conserved residues of the lipase catalytic triad and oxy-anion hole. Among known lipases, PNLIPRP2 was most related to a mouse T-cell lipase, with 74% amino acid identity. PNLIPRP2 has 65% amino acid sequence identity with PNLIP, 63% identity with PNLIPRP1, and 20 to 30% identity with human gastric lipase, lipoprotein lipase, and hepatic lipase.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilutions:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110D359Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human pancreatic lipase, EC number: 3.1.1.3
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.
Specificity:
Recognizes human lipase. Minimal reactivity with other serum proteins.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.