Technical Data
L2601-02B
Lipoprotein Receptor, Low Density (LDLR, LDL Receptor)
Description:
Applications:
Suitable for use in Western Blot, FACS and Immunofluorescence microscopy. Other applications not tested.

Recommended Dilution:
Immunofluorescence microscopy: 1:10-1:50.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O or 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b5F213Affinity Purified
SizeStorageShippingSourceHost
50ug4°C (-20°C Glycerol)Blue IceBovineMouse
Concentration:
As reported
Immunogen:
Purified bovine adrenal cortex LDL receptor.
Purity:
Purified by affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH7.4, 0.5% BSA. Reconstitute with 1ml sterile dH2O or 40-50% glycerol.
Specificity:
Recognizes bovine LDL Receptor. Recognizes an epitope in the region of repeat #1 of the ligand binding region. Detects the 160kD polypeptide in human normal fibroblasts and in bovine adrenal gland. Addition of 15nM antibody results in inhibition of half-maximal LDL-binding. Species Crossreactivity: human . Does not react with rat, mouse, hamster (chinese hamster ovary cells), canine or rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Beisiegel U, Schneider WJ, Goldstein JL, Anderson RGW, Brown MS: Monoclonal antibodies to the low density lipoprotein receptor as probes for study of receptor-mediated endocytosis and the genetics of familial hypercholesterolemia. J Biol Chem 256, 11923-11931 (1981)
2. Beisiegel U, Schneider WJ, Brown MS, Goldstein JL. Immunoblot analysis of low density lipoprotein receptors in fibroblasts from subjects with familial hypercholesterolemia. J Biol Chem 257, 13150-13156 (1982)
3. Schmitz G, Brüning T, Kovacs E, Barlage S. Fluorescence flow cytometry of human leukocytes in the detection of LDL receptor defects in the differential diagnosis of hypercholesterolemia. Arteriosclerosis & Thrombosis 13, 1053-1065 (1993)
4. Virgolini I, Li S-R, yang Q, Koller E, Sperr WR, Leimer M, Angelberger P, Nimpf J, Schneider W, Valent P. Characterization of L and VLDL binding sites on human basophils and mast cells. Arteriosclerosis, Thrombosis & Vascular Biology 15, 17-26 995)
LD(1