Technical Data
L2623-11
LOX (LOX, Lysyl Oxidase, MGC105112, Protein-Lysine 6-Oxidase)
Description:
Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. It plays a vital role in the formation and repair of the extracellular matrix. Also, LOX is a multifunctional enzyme having diverse biological functions such as developmental regulation, tumor suppression, cell motility and cellular senescence. The secreted form of LOX is responsible for the invasive properties of hypoxic human cancer cells. Thus, it is essential for hypoxia-induced metastasis and is a good therapeutic target for preventing and treating metastases.

Applications:
Suitable for use in ELISA, Western Blot, Immunohistochemistry and Immunofluorescence. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:4000; Shows a band at ~33kD.
Immunohistochemistry (Frozen, Paraffin): 1:100-1:250; Stains mouse spleen sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceRatRabbit
Concentration:
Not Determined
Immunogen:
Two synthetic peptides (within aa150-200 and aa275-325) of the rat LOX protein. Cellular Localization: Cytosolic and nuclear.
Purity:
Serum
Form
Suppied as a liquid, 0.1% sodium azide.
Specificity:
Recognizes rat LOX at ~33kD. Species Crossreactivity: mouse, human and primate
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jeay, S., et al., Mol. and Cell Biol. 23(7): 22512263 (2003). 2. Contente S et al., Biochim Biophys Acta 1793(7) 1272-8 (2009)